Summary
Type: SPE
Application #: 303760
Catalog#: 730004 - SPE Cartridges, Chromabond, C18, 6ml, PP, 500mg, 30/pk
Detection: After derivatisation the sample was injected into GC-MS.
Conditions: Column conditioning: The columns were conditioned with 3 mL methanol followed by 3 mL water.
Sample aspiration: The hydrolysed sample was applied onto the C18 cartridge.
Column washing: The column was washed with 3 mL water.
Elution: 1 mL methanol.
Further sample treatment:
The eluate was solvolysed with 5 mL of a mixture of ethyl acetate-sulfuric acid (200 μg H2SO4 in 250 mL ethyl acetate) for 60 min at 60 °C. Following evaporation of the ethyl acetate 750 μL of KOH (1 M) were added. The hydroxylated stanzolol was then extracted into 5 mL of tert.-butyl methylether which was evaporated and dried.
Quantification was achieved by applying a sample spiked with an internal standard.
Authors: M Machnik M Düe M Parr C von Kuk W Schänzer
Source: Chromatographia Supplement 2004 59 S131-S135